extract of azadirachta indica (neem) leaf induces apoptosis in 4t1 breast cancer balb/c mice

نویسندگان
چکیده

objective: azadirachta indica (neem) has been used traditionally for many centuries. some impressive therapeutic qualities have been discovered. however, the therapeutic effect of neem leaf extract in 4t1 breast cancer has not been documented. the purpose of the present study is to investigate the therapeutic effect of ethanolic neem leaf extract in an in vivo 4t1 breast cancer model in mice. materials and methods: a total of 84 female balb/c mice were divided randomly into 7 groups (3 non-cancerous groups and 4 cancerous groups) consisting of 12 mice per group. the 3 non-cancerous groups were normal mice treated with 0.5% of tween 20 in phosphate buffer saline (pbs) (nc), 250 mg/kg neem (n250) or 500 mg/kg neem (n500). the 4 cancerous groups were; cancer controls treated with 0.5% of tween 20 in pbs (cc), and cancerous mice treated with 0.5 µg/ml tamoxifen citrate (ct), 250 mg/kg neem leaf extract (cn 250) or 500 mg/kg neem leaf extract (cn 500). terminal deoxynucleotidyl transferase dutp nick end labeling (tunel) assays were used to evaluate apoptosis (cell death) in the breast cancer tissues. spss software, version 14 was used for statistical analysis. statistical significance was defined as p<0.05. non parametric analysis of variance (anova) was performed with the kruskal wallis test for the tunel assays. parametric data among the groups was compared using anova. results: tunel assays showed that the cn 250 and cn 500 groups had a higher incidence of apoptosis compared with the cancer controls. conclusion: the findings showed that neem leaf extract induces apoptosis in 4t1 breast cancer balb/c mice.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Extract of Azadirachta indica (Neem) Leaf Induces Apoptosis in 4T1 Breast Cancer BALB/c Mice

OBJECTIVE Azadirachta indica (Neem) has been used traditionally for many centuries. Some impressive therapeutic qualities have been discovered. However, the therapeutic effect of neem leaf extract in 4T1 breast cancer has not been documented. The purpose of the present study is to investigate the therapeutic effect of ethanolic Neem leaf extract in an in vivo 4T1 breast cancer model in mice. ...

متن کامل

effect of neem leaf extract (azadirachta indica) on c-myconcogene expression in 4t1 breast cancer cells of balb/c mice

objective: breast cancer is the most common cause of cancer-related deaths in women both worldwide and in malaysia. azadirachta indica (a. juss), commonly known as neem, is one of the most versatile medicinal plants that has gained worldwide prominence due to its medicinal properties. however, the anticancer effect of ethanolic neem leaf extract against breast cancer has not been documented. th...

متن کامل

Effect of Neem Leaf Extract (Azadirachta indica) on c-Myc Oncogene Expression in 4T1 Breast Cancer Cells of BALB/c Mice

OBJECTIVE Breast cancer is the most common cause of cancer-related deaths in women both worldwide and in Malaysia. Azadirachta indica (A. Juss), commonly known as neem, is one of the most versatile medicinal plants that has gained worldwide prominence due to its medicinal properties. However, the anticancer effect of ethanolic neem leaf extract against breast cancer has not been documented. The...

متن کامل

Neem (Azadirachta indica L.) leaf extract deteriorates oocyte quality by inducing ROS-mediated apoptosis in mammals

Neem (Azadirachta indica L.) leaf has been widely used in ayurvedic system of medicine for fertility regulation for a long time. The molecular mechanism by which neem leaf regulates female fertility remains poorly understood. Animal studies suggest that aqueous neem leaf extract (NLE) induces reactive oxygen species (ROS) - mediated granulosa cell apoptosis. Granulosa cell apoptosis deprives oo...

متن کامل

Neem (Azadirachta indica) | Feedipedia

Neem, neem tree, Indian lilac, margosa, nimtree [English]; margousier, margosier, neem [French]; nim, margosa, lila india [Spanish]; amargosa [Portuguese]; Niembaum [German]; mimba [Indonesian]; mwarobaini [Kiswahili]; sầu đâu [Vietnamese]; عئاش مين [Arabic]; [Bengali]; [Burmese]; [Gujarati]; תידוה תכרדזא [Hebrew]; [Hindi]; インドセンダン [Japanese]; [Kannada]; [Marathi]; [Mayalayam]; [Nepalese]; [Pen...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
cell journal

جلد ۱۳، شماره ۲، صفحات ۱۰۷-۱۱۶

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023